AgrobacTransV1, Main, Exploration, bibRecord, 000327

Transient and stable transformation of Ceratopteris richardii gametophytes.

Identifieur interne : 000327 ( Main/Exploration ); précédent : 000326; suivant : 000328

Transient and stable transformation of Ceratopteris richardii gametophytes.

Auteurs : Linh T. Bui [États-Unis] ; Angela R. Cordle [États-Unis] ; Erin E. Irish [États-Unis] ; Chi-Lien Cheng [États-Unis]

Source :

BMC research notes [ 1756-0500 ] ; 2015.

RBID : pubmed:26040630

Descripteurs français

KwdFr :
- Agrobacterium (génétique), Dosage génique (MeSH), Facteurs temps (MeSH), Fougères (croissance et développement), Fougères (génétique), Fougères (métabolisme), Génotype (MeSH), Ovule (botanique) (croissance et développement), Ovule (botanique) (génétique), Ovule (botanique) (métabolisme), Paroi cellulaire (métabolisme), Phénotype (MeSH), Pollen (croissance et développement), Pollen (génétique), Pollen (métabolisme), Prolifération cellulaire (MeSH), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Transduction génétique (méthodes), Transformation génétique (MeSH), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
MESH :
- croissance et développement : Fougères, Ovule (botanique), Pollen, Végétaux génétiquement modifiés.
- génétique : Agrobacterium, Fougères, Ovule (botanique), Pollen, Protéines végétales, Végétaux génétiquement modifiés.
- métabolisme : Fougères, Ovule (botanique), Paroi cellulaire, Pollen, Protéines végétales, Végétaux génétiquement modifiés.
- méthodes : Transduction génétique.
- Dosage génique, Facteurs temps, Génotype, Phénotype, Prolifération cellulaire, Régulation de l'expression des gènes végétaux, Transformation génétique, Vecteurs génétiques.

English descriptors

KwdEn :
- Agrobacterium (genetics), Cell Proliferation (MeSH), Cell Wall (metabolism), Ferns (genetics), Ferns (growth & development), Ferns (metabolism), Gene Dosage (MeSH), Gene Expression Regulation, Plant (MeSH), Genetic Vectors (MeSH), Genotype (MeSH), Ovule (genetics), Ovule (growth & development), Ovule (metabolism), Phenotype (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Pollen (genetics), Pollen (growth & development), Pollen (metabolism), Time Factors (MeSH), Transduction, Genetic (methods), Transformation, Genetic (MeSH).
MESH :
- chemical , genetics : Plant Proteins.
- genetics : Agrobacterium, Ferns, Ovule, Plants, Genetically Modified, Pollen.
- growth & development : Ferns, Ovule, Plants, Genetically Modified, Pollen.
- metabolism : Cell Wall, Ferns, Ovule, Plant Proteins, Plants, Genetically Modified, Pollen.
- methods : Transduction, Genetic.
- Cell Proliferation, Gene Dosage, Gene Expression Regulation, Plant, Genetic Vectors, Genotype, Phenotype, Time Factors, Transformation, Genetic.

Abstract

BACKGROUND

Ferns, being vascular yet seedless, present unparalleled opportunities to investigate important questions regarding the evolution and development of land plants. Ceratopteris richardii, a diploid, homosporous fern has been advanced as a model fern system; however, the tenuous ability to transform the genome of this fern greatly limited its usefulness as a model organism. Here we report a simple and reliable Agrobacterium-mediated method for generating transient and stable transformants of mature C. richardii gametophytes.

RESULTS

Transformation success was achieved by enzyme treatment that partially digested the cell walls of mature gametophytes to facilitate Agrobacteria infection. Co-incubation of Agrobacteria with enzymatically treated gametophytes was sufficient to generate transient transformants at a frequency of nearly 90% under optimal conditions. Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T0 gametophytes on selective media.

CONCLUSIONS

This transformation method will allow for the immediate observation of phenotypes in the haploid gametophytes of transformed plants, as well as the generation of stably transformed C. richardii lines for further analysis. Transformation capability will greatly facilitate gene functional studies in C. richardii, more fully realizing the potential of this model fern species. These protocols may be adapted to other plant species that are recalcitrant to Agrobacterium-mediated transformation.

DOI: 10.1186/s13104-015-1193-x
PubMed: 26040630
PubMed Central: PMC4467839

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000336
to stream Main, to step Curation: 000336

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (genetics)</term>
<term>Cell Proliferation (MeSH)</term>
<term>Cell Wall (metabolism)</term>
<term>Ferns (genetics)</term>
<term>Ferns (growth & development)</term>
<term>Ferns (metabolism)</term>
<term>Gene Dosage (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Ovule (genetics)</term>
<term>Ovule (growth & development)</term>
<term>Ovule (metabolism)</term>
<term>Phenotype (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Pollen (genetics)</term>
<term>Pollen (growth & development)</term>
<term>Pollen (metabolism)</term>
<term>Time Factors (MeSH)</term>
<term>Transduction, Genetic (methods)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (génétique)</term>
<term>Dosage génique (MeSH)</term>
<term>Facteurs temps (MeSH)</term>
<term>Fougères (croissance et développement)</term>
<term>Fougères (génétique)</term>
<term>Fougères (métabolisme)</term>
<term>Génotype (MeSH)</term>
<term>Ovule (botanique) (croissance et développement)</term>
<term>Ovule (botanique) (génétique)</term>
<term>Ovule (botanique) (métabolisme)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Phénotype (MeSH)</term>
<term>Pollen (croissance et développement)</term>
<term>Pollen (génétique)</term>
<term>Pollen (métabolisme)</term>
<term>Prolifération cellulaire (MeSH)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Transduction génétique (méthodes)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (MeSH)</term>
<term>Végétaux génétiquement modifiés (croissance et développement)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
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<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Fougères</term>
<term>Ovule (botanique)</term>
<term>Pollen</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium</term>
<term>Ferns</term>
<term>Ovule</term>
<term>Plants, Genetically Modified</term>
<term>Pollen</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ferns</term>
<term>Ovule</term>
<term>Plants, Genetically Modified</term>
<term>Pollen</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium</term>
<term>Fougères</term>
<term>Ovule (botanique)</term>
<term>Pollen</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term>
<term>Ferns</term>
<term>Ovule</term>
<term>Plant Proteins</term>
<term>Plants, Genetically Modified</term>
<term>Pollen</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Transduction, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fougères</term>
<term>Ovule (botanique)</term>
<term>Paroi cellulaire</term>
<term>Pollen</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Transduction génétique</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Proliferation</term>
<term>Gene Dosage</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genetic Vectors</term>
<term>Genotype</term>
<term>Phenotype</term>
<term>Time Factors</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Dosage génique</term>
<term>Facteurs temps</term>
<term>Génotype</term>
<term>Phénotype</term>
<term>Prolifération cellulaire</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Transformation génétique</term>
<term>Vecteurs génétiques</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Ferns, being vascular yet seedless, present unparalleled opportunities to investigate important questions regarding the evolution and development of land plants. Ceratopteris richardii, a diploid, homosporous fern has been advanced as a model fern system; however, the tenuous ability to transform the genome of this fern greatly limited its usefulness as a model organism. Here we report a simple and reliable Agrobacterium-mediated method for generating transient and stable transformants of mature C. richardii gametophytes.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Transformation success was achieved by enzyme treatment that partially digested the cell walls of mature gametophytes to facilitate Agrobacteria infection. Co-incubation of Agrobacteria with enzymatically treated gametophytes was sufficient to generate transient transformants at a frequency of nearly 90% under optimal conditions. Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T0 gametophytes on selective media.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>This transformation method will allow for the immediate observation of phenotypes in the haploid gametophytes of transformed plants, as well as the generation of stably transformed C. richardii lines for further analysis. Transformation capability will greatly facilitate gene functional studies in C. richardii, more fully realizing the potential of this model fern species. These protocols may be adapted to other plant species that are recalcitrant to Agrobacterium-mediated transformation.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26040630</PMID>
<DateCompleted><Year>2016</Year>
<Month>02</Month>
<Day>25</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1756-0500</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<PubDate><Year>2015</Year>
<Month>Jun</Month>
<Day>04</Day>
</PubDate>
</JournalIssue>
<Title>BMC research notes</Title>
<ISOAbbreviation>BMC Res Notes</ISOAbbreviation>
</Journal>
<ArticleTitle>Transient and stable transformation of Ceratopteris richardii gametophytes.</ArticleTitle>
<Pagination><MedlinePgn>214</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s13104-015-1193-x</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Ferns, being vascular yet seedless, present unparalleled opportunities to investigate important questions regarding the evolution and development of land plants. Ceratopteris richardii, a diploid, homosporous fern has been advanced as a model fern system; however, the tenuous ability to transform the genome of this fern greatly limited its usefulness as a model organism. Here we report a simple and reliable Agrobacterium-mediated method for generating transient and stable transformants of mature C. richardii gametophytes.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Transformation success was achieved by enzyme treatment that partially digested the cell walls of mature gametophytes to facilitate Agrobacteria infection. Co-incubation of Agrobacteria with enzymatically treated gametophytes was sufficient to generate transient transformants at a frequency of nearly 90% under optimal conditions. Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T0 gametophytes on selective media.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This transformation method will allow for the immediate observation of phenotypes in the haploid gametophytes of transformed plants, as well as the generation of stably transformed C. richardii lines for further analysis. Transformation capability will greatly facilitate gene functional studies in C. richardii, more fully realizing the potential of this model fern species. These protocols may be adapted to other plant species that are recalcitrant to Agrobacterium-mediated transformation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bui</LastName>
<ForeName>Linh T</ForeName>
<Initials>LT</Initials>
<AffiliationInfo><Affiliation>Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA. lbui@danforthcenter.org.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cordle</LastName>
<ForeName>Angela R</ForeName>
<Initials>AR</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Iowa, Iowa City, IA, 52242, USA. angela-cordle@uiowa.edu.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Irish</LastName>
<ForeName>Erin E</ForeName>
<Initials>EE</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Iowa, Iowa City, IA, 52242, USA. erin-irish@uiowa.edu.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cheng</LastName>
<ForeName>Chi-Lien</ForeName>
<Initials>CL</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Iowa, Iowa City, IA, 52242, USA. chi-lien-cheng@uiowa.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
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<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>06</Month>
<Day>04</Day>
</ArticleDate>
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<MedlineTA>BMC Res Notes</MedlineTA>
<NlmUniqueID>101462768</NlmUniqueID>
<ISSNLinking>1756-0500</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029624" MajorTopicYN="N">Ferns</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018628" MajorTopicYN="N">Gene Dosage</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056771" MajorTopicYN="N">Ovule</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014161" MajorTopicYN="Y">Transduction, Genetic</DescriptorName>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

Transient and stable transformation of Ceratopteris richardii gametophytes.

Transient and stable transformation of Ceratopteris richardii gametophytes.

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